Abstract
Homozygous nude and normal mice of 3 strains, BALB/c, CBA and C57BL, were used as sources of nucleated haemopoietic "natural killer" (NK) cells. These killer cells could lyse a wide range of syngeneic and allogeneic lymphoid tumour cell lines in vitro, and it was found that cell suspensions from nude mice were always significantly more active than those from normal mice, and that the most active effector population was a polymorph-enriched peritoneal-exudate cell suspension. Eosinophils did not appear to be involved in the phenomenon, and mononuclear peritoneal-exudate cell suspensions were actually highly inhibitory. Three non-lymphoid tumours, a carcinoma, a fibrosarcoma and a mastocytoma, were totally resistant to in vitro lysis. Although all susceptible tumour cell lines were C-type virus-associated, not all of these tumours were killed by all strain sources of spleen cells, indicating a specificity of killing.
Highlights
Tumour Strain of origin TypeH-2 type 51Cr release*WEHI-7 WEHI-22 ABE-8 WEHI-265 WEHI-164 MPC- 11 EMT-6 HPC-10 P815 EL4 C1.18 RILQ BALB/c T lymphoma d d
The natural killer" (NK) activity of spleen cells from BALB/c. nu mice for the T lymphoma WEHI-7 is represented in Fig. 1, with CL/T ratios of 200/1-12.5/1
CL/T ratios of 200/1 and 12.5/1 gave low levels of cytotoxicity, and the peak level was almost invariably at 100/1 or 50/1. These are not distinguished in the figure a number of experiments were performed using agematched 5-8-week-old male and female BALB/c nudes
Summary
The NK activity of spleen cells from BALB/c. nu mice for the T lymphoma WEHI-7 is represented in Fig. 1, with CL/T ratios of 200/1-12.5/1. Nu mice for the T lymphoma WEHI-7 is represented, with CL/T ratios of 200/1-12.5/1. There was a wide day-to-day variation in the cytotoxicity measured over 20 experiments. For any particular experiment a CL/T curve of the form illustrated for a representative sample of experiments was always found. CL/T ratios of 200/1 and 12.5/1 gave low levels of cytotoxicity, and the peak level was almost invariably at 100/1 or 50/1. These are not distinguished in the figure a number of experiments were performed using agematched 5-8-week-old male and female BALB/c nudes. There was no significant difference in the cytotoxicity, as measured by %O specific lysis of 51Cr-labelled WEHI-.
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