Native isolate of Trichoderma: a biocontrol agent with unique stress tolerance properties

Abstract

Species of Trichoderma are widely recognized for their biocontrol abilities, but seldom studied collectively, for their plant growth promotion, abiotic stress tolerance and bioremediation properties. Our study is a concentrated effort to establish the potential of native isolate Trichoderma harzianum KSNM (T103) to tolerate biotic (root pathogens) and abiotic stresses [high salt (100-1000mM); heavy metal (chromium, nickel and zinc: 1-10mM); pesticides: malathion (100-600ppm), carbofuran (100-600ppb)], along with its ability to support plant growth. In vitro growth promotion assays with T103 treated Vigna radiata, Vigna mungo and Hordeum vulgare confirmed 'non-species specific' growth promotion effects of T103. At lower metal concentration, T103 treatment was found to completely negate the impact of metal stress [60% increase in radicle length (RL) with no significant decrease in %germination (%G)]. Even at 10mM metal, T103 inoculation gave 80% increase in %G and >50% increase in RL. In vitro experiments confirmed high metal reduction capacity (47%-Cr, 35%-Ni and 42%-Zn) of T103 at concentrations as high as 4mM. At maximum residual concentrations of malathion (440ppm) and carbofuran (100ppb) reported in agricultural soils, T103 maintained 80 and 100% survivability, respectively. T103 treatment has improved %G and RL in all three hosts challenged with pesticide. Isolate T103 was found to effectively suppress growth of three major root pathogens: Macrophomina phaseolina (65.83%) followed by Sclerotium rolfsii (19.33%) and Fusarium oxysporum (19.18%). In the light of these observations, native T. harzianum (T103) seems to be a competent biocontrol agent for tropical agricultural soils contaminated with residual pesticides and heavy metals.