Nanoscale virus biosensors: state of the art

Abstract

Nanoscale virus biosensors: state of the art Ludmila Krejcova,1 Petr Michalek,2 Miguel Merlos Rodrigo,2 Zbynek Heger,2 Sona Krizkova,1 Marketa Vaculovicova,1 David Hynek,1 Vojtech Adam,1 Rene Kizek1 1Central European Institute of Technology, Brno University of Technology, 2Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University in Brno, Brno, Czech Republic Abstract: Since the beginning of the new millennium, viruses have shown huge epidemiological and pandemic potential: severe acute respiratory syndrome (SARS) in 2002, pandemic swine flu in 2009, and last but not least the West African Ebola outbreak in 2014. The occurrence and spread of the new virus in pandemic dimension poses a threat to the health and lives of 7 billion people worldwide. There is a growing urgency for highly sensitive and selective detection techniques, usable for a wide number of applications, including disease diagnosis, pharmaceutical research, agriculture, as well as preventive measures. Nanobiosensors represent a new promising tool for virus detection. This review gives a brief survey of the issue of viral detection, comprising diagnostics of target structure of viruses such as nucleic acids or proteins. This review covers different detection principles, methods of fabrication, and applications of virus biosensors. Keywords: nanoparticle, nanomaterial, diagnostics, immunosensors, hybridization&nbsp