Abstract
A protein patterned surface using micro-contact printing methods has been investigated byscanning force microscopy. Electrostatic force microscopy (EFM) was utilized for imaging thetopography and detecting the electrical properties such as the local bound charge distributionof the patterned proteins. It was found that the patterned IgG proteins are arranged down to1 µm, andthe 90° rotation of patterned anti-IgG proteins was successfully undertaken. Through theestimation of the effective areas, it was possible to determine the local boundcharges of patterned proteins which have opposite electrostatic force behaviors.Moreover, we studied the binding probability between IgG and anti-IgG in a1 µm2 MIMIC system by topographic and electrostatic signals for applicable label-free detections.We showed that the patterned proteins can be used for immunoassay of proteins on thefunctional substrate, and that they can also be used for bioelectronics device application,indicating distinct advantages with regard to accuracy and a label-free detection.
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