Abstract
Purpose: Chitosan is a natural mucoadhesive polymer with antibacterial activity. In the present study, chitosan (CS) nanoparticles were investigated as a vehicle for delivery of antibiotic, ciprofloxacin hydrochloride.Methods: Ionotropic gelation method was used for preparation chitosan nanoparticles. The effects of various factors including concentration of CS, concentration of tripolyphosphate (TPP), and homogenization rate on the size of nanoparticles were studied. The effects of various mass ratios of CS to ciprofloxacin hydrochloride on the encapsulation efficiency of nanoparticles were assessed.Results: The particles prepared under optimal condition of 0.45% CS concentration, 0.45% TPP concentration and homogenizer rate at 6000 rpm, had 72 nm diameter. In these particles with 1:0.5 mass ratio of CS to ciprofloxacin hydrochloride, the encapsulation efficiency was 23%. The antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded nanoparticles against E.coli and S.aureus was evaluated by calculation of minimum inhibitory concentration (MIC). Results showed that MIC of ciprofloxacin loaded chitosan nanoparticles was 50% lower than that of ciprofloxacin hydrochloride alone in both of microorganism species. Nanoparticles without drug exhibited antibacterial activity at higher concentrations and MIC of them against E.coli and S.aureus was 177 and 277 µg/ml, respectively.Conclusion: Therefore chitosan nanoparticles could be applied as carrier for decreasing the dose of antibacterial agents in the infections.
Highlights
Management of infectious disease can be improved by prolonging the contact time of antibiotics with the microorganism surface
The antibacterial activity of chitosan nanoparticles and ciprofloxacin-loaded nanoparticles against E.coli and S.aureus was evaluated by calculation of minimum inhibitory concentration (MIC)
In the present work we developed ciprofloxacin-loaded chitosan nanoparticles and evaluated their physicochemical properties
Summary
Management of infectious disease can be improved by prolonging the contact time of antibiotics with the microorganism surface. Deoxy-d-glucose] as a poly cationic biopolymer has high antimicrobial activity.[3,4] This natural polysaccharide possesses useful properties such as non-toxicity, biodegradability, low price, high biocompatibility and non-antigenicity.[3,4,5,6,7,8,9,10] The proposed mechanism for its antimicrobial action is binding to the negatively charged bacterial cell wall, with consequent destabilization of the cell envelope and altered permeability, followed by attachment to DNA with inhibition of its replication.[1,11,12]. Through its positive ionic interactions with the negative charges of the cell surface membranes the drug can be exposed to microorganisms for a longer time.[11,13,14] it has been shown that chitosan and its derivatives can act as antibacterial agents against both Gram-negative and Gram-positive bacteria.[14]
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