Abstract
Herein, Piperine, a phytochemical present in Long pepper is quantitatively analyzed via an electrochemical technique using a chemically modified electrode. Mesoporous nanomaterial has been utilized as a base matrix to carry out the experiments. The piperine is isolated from crude Long pepper through a standard procedure. Glassy carbon electrode is chemically modified with mesoporous carbon matrix and isolated piperine designated as (GCE/GMC@piperine). The cyclic voltammetry response gave a perfect redox response of piperine at E’= +0.2 V vs Ag/AgCl at 50 mV s-1in pH 7 PBS. Effect of scan rate and effect of solution pH was studied. Further, it was observed that a change in concentration of piperine is directly proportional to the redox peak current obtained. Therefore, this studies could act as a key for quantitative analysis of Piperine; naturally occurring phytochemical in natural products like Pepper, Long pepper, white pepper etc. This is a prototype study and can be further extended to disposable screen printed electrodes for portable analysis.
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