Nanofibrous Wound Healing Nanocomposite Based on Alginate Scaffold: In Vitro and In Vivo Study

Abstract

The combination of nanofibers with 3D scaffolds has shown promising results as the wound healing/dressing/care biomaterials. The present study aimed to fabricate and optimized alginate hydrogel composited by Lignin-derived carbon nanofibers (CNFs). The nanofibers were obtained from electrospun Lignin nanofibers as the precursor through two steps heat treatments. The synthesized nanofibers blended with an alginate polymer solution with different concentrations (1, 5, and 10 wt.%) and cross-linked using CaCl2 through the physical cross-linking. The findings illustrated that the prepared Lignin and CNFs have acceptable diameter. The composited Alginate hydrogels possessed a porous internal-structure with interconnected architecture. The fabricated hydrogel exhibited proper porosity and swelling behavior beneficial for wound healing application. The In Vitro experiments revealed that the hydrogel were red blood cell (RBC)-compatible, cytocompatible, and induced proliferative effects on cells. The animal experiments indicated that the application of the hydrogel promoted the process of wound healing. These observations implied that the prepared hydrogel nanocomposites exhibited promising properties and can be considered as wound healing nanobiomaterials.