Nanofertilizers and plant metabolites: Investigating their influence on trophic interactions in the cucumber-melon aphid-green lacewing

Abstract

The role of fertilizers in increasing nutritional content and making host plants more attractive to herbivores has been repeatedly proved. This study investigated the effect of applying three levels of nano-chelated fertilizer (vegetables and summer crops specific fertilizer) (2, 3, and 4 kg/ha) on the levels of malondialdehyde (MDA), and phenylalanine ammonia-lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO), catalase (CAT), ascorbic acid (ASA), flavonoid, and phenolic compounds in cucumber plant (Cucumis sativus L.). Additionally, the research surveyed the influence of fertilization and cucumber metabolite compounds on the life history of Aphis gossypii (Glover) (Hemiptera: Aphididae) and Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) in a tri-trophic context. Host plant content analysis showed that biotic stress resulting from the aphid infestation in cucumber plants increased the POD, PPO, CAT activity, and MDA contents. However, it decreased PAL activity, flavonoid, and phenol contents in the control treatment and 2, 3, and 4 kg/ha of nano-chelated fertilizer levels. Moreover, plant fertilization increased the herbivore population growth rates and decreased the predator population growth rates by affecting the nutritional contents and quality of the host plant. Accordingly, the aphid's intrinsic rate of increase (r) was ranged from 0.319 to 0.365 day−1. While finite rate of increase (λ) was estimated as 1.376 to 1.441 day−1 on control to 2 kg/ha nano-chelated fertilizer treatment. In addition, the intrinsic rate of increase of C. carnea was 0.116 to 0.131 and the finite rate of increase was 1.123 to 1.139 day−1 on 2 kg/ha nano-chelated fertilizer and control treatments, respectively. The maximum and minimum net reproductive rate (R0) values of the aphid were recorded on 3 kg/ha nano-chelated fertilizer and control treatments as 46.34 and 37.18 offsprings, respectively, whilst these values ranged from 145.51 on 2 kg/ha to 196.93 offsprings on 4 kg/ha regimes for the green lacewing. Notably, the utilization of 2, 3, and 4 kg/ha of nano-chelated fertilizer promotes cucumber defensive metabolites compounds such as PAL, POD, CAT, flavonoid, and ASA and induces the plant's resistance to melon aphids directly. It can be concluded that the utilization of nano-chelated fertilizer potentially can enhance cucumber plant resistance to A. gossypii by improving the antioxidant metabolite compounds, and be an effective part of integrated pest management programs.