Abstract
The procedure for the fabrication of a detector termed NACAD, nanofabricated carbon-based detector, is described. I characterized the mechanical properties of this detector using atomic force and scanning electron microscopy techniques. This detector exhibits a smooth surface (rms 2-4 nm) and easy access for atomic force microscopy tips, necessary features for biological studies of secretory granules and vesicles, which store transmitters and release them via exocytosis. The NACAD does not impair the elastic properties of granular matrixes deposited onto a detector, as they show their typical Young's moduli and ion exchanger properties; divalent histamine shrinks them, while monovalent sodium causes their swelling. Additionally, the NACADs' electrochemical properties allowed amperometric measurements of serotonin released from intact secretory granules isolated from mast cells, after removal of their granular membranes using a mild detergent treatment. Thus, this detector will aid future studies of single secretory granules and vesicles and their insoluble matrixes.
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