Abstract
Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgGI125 and RAM-nanodiamond-BSAI125 complexes are stable in blood serum and the immobilized proteins retain their biological activity. It was shown that the RAM-nanodiamond-BSAI125 complex is able to bind to the target antigen immobilized on the Sepharose 6B matrix through antibody–antigen interaction. The idea can be extended to use nanodiamonds as carriers for delivery of bioactive substances (i.e., drugs) to various targets in vivo.
Highlights
Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody
It was shown that the Rabbit Anti-Mouse Antibody (RAM)-nanodiamond-BSAI125 complex is able to bind to the target antigen immobilized on the Sepharose 6B matrix through antibody–antigen interaction
We investigated the possibility of conjugating nanodiamonds with iodine-125 labeled immunoglobulin (IgGI125) and bovine serum albumin (BSAI125), and Rabbit Anti-Mouse Antibody (RAM), as well as properties of the obtained complexes
Summary
Surface of detonation nanodiamonds was functionalized for the covalent attachment of immunoglobulin, and simultaneously bovine serum albumin and Rabbit Anti-Mouse Antibody. The nanodiamond-IgGI125 and RAM-nanodiamond-BSAI125 complexes are stable in blood serum and the immobilized proteins retain their biological activity. The precipitate was repeatedly washed with the PBS-buffer to remove the unbound protein, every time re-suspending the nanoparticles in a fresh buffer, collecting them by centrifugation under the above-mentioned conditions and taking a supernatant for radioactivity evaluation.
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