Nano copper oxide-incorporated mesoporous carbon composite as multimode adsorbent for selective isolation of hemoglobin.

Abstract

Assembly of nano-objects with tunable size, morphology and function into integrated nanostructures is critical for the development of a novel nanosystem in adsorption, sensing and drug/gene delivery. We demonstrate herein the fabrication of ordered mesoporous carbon by assembling uniform and highly dispersed copper-oxide (CuxOy) nanoparticles into the mesopores via evaporation of solvent from the mixture of triblock copolymer, carbon source and metal nitrate hydrate. The ordered 2D hexagonal mesoporous carbon composite possesses a large surface area of 580.8 cm(2)/g, a uniform pore size of 5.4 nm, a large pore volume of 0.64 cm(3)/g and a high metal content of 3.32 wt %. The mesoporous composite exhibits excellent adsorption selectivity and high adsorption capacity to hemoglobin (Hb) under the synergistic effect of hydrophobic and metal-affinity interactions as well as size exclusion. This facilitates multimode adsorption of hemoglobin fitting Langmuir adsorption model and offers an adsorption capacity of 1666.7 mg g(-1) for hemoglobin. The mesoporous composite is used for the isolation of hemoglobin from human whole blood with high purity. It demonstrates the potential of the copper-oxide nanoparticle-embedded mesoporous carbon composite in selective isolation/removal of specific protein species from biological sample matrixes.