Nano carbon water retaining fertilizer on soil bacterial community structure and grape growth in grape field

Abstract

In order to study the application of nano carbon water retaining fertilizer in agricultural production, and to explore the effects of its use on the bacterial community structure of agricultural soil and crop growth, a series of experimental studies were carried out in a grape field. In this paper, the coated slow-release fertilizer with soluble nitrogen particles as the core and nano carbon materials wrapped in the outer layer of nitrogen fertilizer was developed. The grape field used in the experiment was divided into experimental field and control field. The new nano carbon water retaining fertilizer was applied in the experimental field and ordinary nitrogen fertilizer was applied in the control field. Through Illumina Miseq high-throughput sequencing, the diversity and richness of bacterial communities in the original soil without planting and the soil of two groups of grape fields after grape ripening were detected, and the grape leaf area, single fruit weight and horizontal diameter of grape, and the titratable acid content of grape fruit in the two fields were detected and calculated. The results showed that Shannon index and Chao1 index of soil bacterial community diversity in the experimental field were higher than those in the control field; the grape leaf area in the experimental field was larger than that in the control field in the early, middle and late stages of shoot growth, with a difference of 8.56cm2 in the later stage; the single fruit weight and transverse diameter of grape in the experimental field were 1.94 g and 2.72 mm larger than those in the control field at 100 days after flowering, respectively; The difference of titratable acid content in grape fruit was the largest at 70 days after anthesis, which was 0.25% and 0.05% at 100 days after flowering. The results showed that nano carbon water retaining fertilizer had significant effects on soil community structure and grape growth in grape field, which could effectively reduce the influence of planting and fertilization on soil bacterial community diversity and richness, promote the growth of grape leaves and fruits, and improve grape quality.