Abstract
Abstract CD3 − CD4 + CD45 + cells are thought to be an inducer cell population for Peyer's patch (PP) and lymph node development. Nasopharyngeal-associated lymphoid tissue (NALT) is an organized lymphoid structure found on both sides of the nasopharyngeal duct dorsal to the cartilaginous soft palate. Although NALT is considered to be functionally analogous to PP in the intestinal tract, the association of CD3 − CD4 + CD45 + cells with the initiation of NALT development has not yet been clarified. To examine this issue further, we isolated CD3 − CD4 + CD45 + cells from the fetal intestines of wild-type mice. Id2 −/− mice genetically deficient in NALT were adoptively transferred with purified CD3 − CD4 + CD45 + cells or fetal liver cells. After the adoptive transfer, we employed immunohistochemical analysis to examine the expression of PNAd, an important addressin for the recruitment of lymphocytes, in the nasal tissue of Id2 −/− mice transferred with fetal liver cells. Though NALT-like structures were indeed induced in Id2 −/− mice adoptively transferred with CD3 − CD4 + CD45 + cells or fetal liver cells, those structures were not observed to express PNAd. These findings indicate that CD3 − CD4 + CD45 + cells and Id2 genes are key to the initiation of NALT. However, they also suggest that an additional element is required for the induction of PNAd.
Published Version
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