Abstract
Summary We have obtained NaCl-tolerant cell lines by exposing tobacco cell suspensions to increasing levels of NaCl. Tolerance to 8.8 g/l NaCl is the maximum obtainable in cell suspensions. Normal lines are tolerant to about 1.6 g/l NaCl. Plants regenerated from resistant cell lines transmit tolerance to two subsequent generations. The level of NaCI-resistance in regenerated plants is higher than that of cell cultures. Few nontolerant F2 plants survive watering with solutions containing more than 15.4 gil NaCI whereas most tolerant plants survive 33.4 g/l NaCl.
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