Abstract
The Na+-H+-exchanger is an essential part in cellular pH-regulation (pHi) and volume control. The objective of the present study was to investigate its activity after acute and chronic acid loading. In part I of the study 16 healthy volunteers performed an incremental treadmill exercise test until exhaustion. Blood was taken before and 1 hour after the test. In part II of the study 8 healthy volunteers performed an anaerobic training for 5 days consisting of 4 × 400 m runs per day (2 in the morning; 2 in the afternoon). Blood was taken at day 1 before training and the day after the last training unit. Lymphocytes were isolated using density-gradient centrifugation. Intracellular pH was measured using the pH sensitive fluorescent indicator BCECF. Cells were acidified using propionic acid and the pHi recovery was investigated. Mean resting pHi of lymphocytes isolated before exercise was 7,26 ± 0,01. Exercise did not affect resting pHi. In contrast, the recovery of pH after acidification of lymphocytes was significantly enhanced after both the treadmill test and the 5 day anaerobic training. These results indicate that acid-loading during anaerobic exercise increases Na+-H+-exchanger activity in human lymphocytes. Moreover, a single bout of exercise is sufficient to induce an exchanger adaptation and after anaerobic training this adaptation lasts for at least 24 hours after the last training unit.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call