Abstract
N-Hydroxysuccinimidyl-3-indolylacetate (SIIA) was firstly synthesized in a very simple route and used as a pre-column derivatizing reagent, which can react with amino compounds selectively under mild conditions. Its derivatives of peptides and their hydrolysates were separated and determined by reversed-phase high performance liquid chromatography (HPLC). In a mobile phase of methanol-water (17:83, v:v) containing 10 mmol/L citric acid-sodium phosphate buffer (pH 3.8), the derivatives of glutathione (GSSG), glycyl glycine (Gly-Gly), glutamic acid (Glu), cystine ((Cys)
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