フラボノイド分析によるハルサザンカとその近縁種との類縁関係の究明

Abstract

A chromatographic study of methanol-soluble flavonoid compounds in mature leaves was undertaken to analyze the phylogenetic relationships among the cultivars and strains of Camellia vernalis (triploid, tetraploid and pentaploid group), C. japonica, C. sasanqua and Stewartia pseudo-camellia. The majority of experimental materials were collected from Hirado Island, Nagasaki prefecture. The former species have been assumed to be hybrids between the second and third species. The results obtained were summarized as follows.:Thirty-nine kinds of spots were detected in the chromatograms of the three species of Camellia and S. pseudo-cammelia used in this study and compared with each other in appearance. Since the peculiar spot patterns on the chromatograms occurred only in C. japonica and C. sasanqua, we examined the spot pattern in C. vernalis and compared it with that of C. japonica and C. sasanqua. A close relation was noted within the triploid group of C. vernalis and C. japonica, and within the tetraploid group of C. vernalis and C. sasanqua. On the other hand, no corresponding connection between the above-mentioned groups was noticed except for two cases.Numerical analyses of the data obtained clearly indicated that the hybrid character of C. vernalis originated from hybridization between C. japonica and C. sasanqua. Also, the affinity values suggested that the triploid group of C. vernalis showed a higher affinity to C. japonica than to its own tetraploid group. On the other hand, numerical treatment of S. pseudo-camellia revealed a heterogenous relation in comparison with the species of Camellia.