硼添加对铝胁迫栝楼生长和生理的影响

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 硼添加对铝胁迫栝楼生长和生理的影响 DOI: 10.5846/stxb202202220419 作者: 作者单位: 作者简介: 通讯作者: 中图分类号: 基金项目: 国家自然科学基金（32001224，41702181）；2021年国家级大学生创新创业训练计划（202110345017） Effects of exogenous boron on growth and physiology of Trichosanthes kirilowii Maxim. under aluminum stress Author: Affiliation: Fund Project: The National Natural Science Foundation of China (General Program, Key Program, Major Research Plan)，the 2021 National Undergraduate Training Program for Innovation and Entrepreneurship 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:为揭示硼对缓解栝楼（Trichosanthes kirilowii Maxim.）铝（Aluminum）毒害的生理机制，以耐铝性强的安国栝楼和耐铝性弱的浦江栝楼为材料，研究了50 μmol/L硼酸（H3BO3）对300 μmol/L Al3+胁迫下栝楼幼苗生长、铝积累、抗氧化能力和细胞壁组分的影响。结果表明：铝胁迫下，植株的根长、株高、鲜重和干重降低，超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）活性受到显著抑制，安国栝楼、浦江栝楼根尖丙二醛（MDA）含量分别增加256.1%、278.2%，细胞壁多糖含量、果胶甲酯酶（PME）活性、根尖铝积累量显著提高，果胶甲基酯化度（DM）和3-脱氧-D-甘露-辛酮糖酸（KDO）含量降低。外源硼可以缓解铝胁迫对栝楼幼苗生长的抑制作用，安国与浦江栝楼的抗氧化酶活性显著提升，MDA含量、细胞壁多糖含量、PME酶活性均降低，安国栝楼果胶DM值恢复至正常水平的91.5%，浦江栝楼KDO含量较单铝处理组上升52.0%，活性铝结合位点减少，有效降低了根尖铝含量并维持其形态结构。因此，硼信号能通过调节细胞壁多糖组分且稳定其网格结构，减少根尖对铝的吸收，同时增强抗氧化酶活性，缓解铝胁迫导致栝楼幼苗生长中的次生氧化胁迫，提高栝楼幼苗对铝毒环境的耐受性，且对耐铝性强的安国栝楼作用效果较好。研究结果可为改良酸铝胁迫下作物的生长环境提供理论指导。 Abstract:Aluminum toxicity, a major abiotic stress that inhibits plant growth and development, has become one of the main factors reducing agricultural yield. To reveal the physiological mechanism of boron on alleviating aluminum toxicity of Trichosanthes kirilowii Maxim., seedlings from Anguo (with strong Al-tolerance) and Pujiang (with weak Al-tolerance) varieties of T. kirilowii were selected for water culture experiments and the effects of boron treatment (50 μmol/L) on varieties' growth, aluminum accumulation, antioxidant capacity, and cell wall components under aluminum stress (300 μmol/L) were studied. The results showed that 300 μmol/L Al3+ stress inhibited the root length, plant height, fresh weight, and dry weight of the plant. The activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) were significantly decreased, and the content of malondialdehyde (MDA) in root tip of T. kirilowii in Anguo and Pujiang varieties increased by 256.1% and 278.2%, respectively. Aluminum stress also significantly enhanced the content of cell wall polysaccharide, pectin methylesterase (PME) activity, and root tip aluminum accumulation. However, the degree of pectin methyl-esterification (DM) and the content of 3-deoxy-d-mannose-octanoic acid (KDO) were strongly inhibited. Exogenous boron could alleviate the inhibitory effect of aluminum stress on the growth of T. kirilowii seedlings. The antioxidant enzyme activities of T. kirilowii in Anguo and Pujiang varieties were increased significantly. The MDA content, cell wall polysaccharide content, and PME enzyme activity were decreased. In addition, the pectin DM value of Trichosanthes in Anguo variety returned to 91.5% of the normal level, and the KDO content of T. kirilowii in Pujiang variety increased by 52.0% compared with that in the single aluminum treatment group. As a result, the active aluminum binding sites decreased, effectively reducing the aluminum content in the root tip and maintaining its morphological structure. Therefore, boron improved the tolerance of T. kirilowii seedlings to aluminum toxic environment by regulating the polysaccharide components of cell wall and stabilizing its grid structure to reduce the absorption of aluminum by root tips, and enhancing the activity of antioxidant enzymes to alleviate the secondary oxidative stress in the growth of T. kirilowii seedlings, which has a better effect on Anguo variety with stronger tolerance to aluminum toxicity. The research in this paper can provide theoretical guidance for improving the environment for crop growth under acid-aluminum stress. 参考文献 相似文献 引证文献