Abstract
Maltooligosaccharides (G3-G6) have been used as reagents for research and clinical reagents for the determination of serum amylase activity. G5 has been used as a nutrient for calorie deprivation. G5 as well as G3, G4, and G6 are all highly soluble and produce clear viscous solutions which are palatable. Therefore, these compounds may potentially be of use as superior nutrients for infants and the aged. The price of pure maltooligosaccharides is, however, extremely high, because of the difficulties encountered in producing each in a pure state . Several reports have been published on the production of G2, G3, G4, G5, etc., from starch. G2 production from starch has been widely studied using plant β-amylases ; G3-forming amylaseswere discovered in various bacteria. Among the maltooligosaccharide (G3-G5)-forming amylases, it was reported that a G4-forming amylase was produced by Pseudomonas stutzeri, but no other bacteria which would produce G 4-forming amylases have yet been reported. Pseudomonas saccharophila was found to produce that enzyme. Detail on this enzyme will be reported in this journal in the near future. G5-forming amylases and a G5-producing bacterium were reported to form mainly G5, with considerable amounts of other oligosaccharides such as G6, G7, G2, G3, and G4, during the reaction and cultivation. The authors tried to find a novel enzyme which forms G5, without any other saccharides at some stage of the reaction. A novel microorganism which produces such an enzyme has been discovered.
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