Abstract
We have succeeded in injection of plasmid to adherent cells which are suspended in the plasma-bubbles laden circulation flow in a chamber. High-speed plasma-bubbles which include plasma induced radical or chemically active species are generated by glass electrode and the air-liquid interface has a stiction force which draws the gene (plasmid) and stick to the air-liquid interface. The circulating flow increased the chance for cells to contact air-liquid interface of bubbles. The plasma-bubble surface has high reactivity due to the plasma induced species and makes it possible to have pore formation on cells membrane, and gene transfer is successfully completed. This technology of two-dimensional microfluidic chip contributes an option to the high-throughput gene transfer.
Published Version
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