Abstract

1. Fungal amylases 1) Aspergillus awamori : Alpha-amylase has an extremely weak activity to digest raw starch, but glucoamylase has strong activity to digest raw starch. Glucoamylase I, can adsorb on raw starch, is the principle of raw starch digestion. Glucoamylase II, can not adsorb on raw starch, has an extremely weak activity to digest raw starch. 2) Rhizopus sp. and Aspergillus oryzae: These two fungi have the similar amylase system with those of Asp, awamori on raw starch digestion and raw starch adsorption. 2. Comparison of four kinds of α-amylase 1) Pancreactic α-amylase digests raw starch most strongly and can adsorb on raw starch most easily. Asp, oryzae α-amylase digests raw starch most weakly and adsorbs on raw starch imperceptibly. In the case of α-amylases from bacteria and malt, α-amylase adsorption curves give reverse position from those shown for digestion. Apparently the ability to adsorb on raw starch does not necessarily indicate ability to digest the raw starch. 2) Glucose and maltose, especially the latter, inhibit both amylase adsorption on raw starch and raw starch digestion, and so the raw starch digestion is accelerated by dialysis. 3. Debranehing enzymes 1) Pseudomonas isoamylase: This enzyme, can adsorb on raw starch, assists the raw starch, especially waxy starch, digestion by Aspi awamori glucoamylase I or II. 2) Aerobacter pullulanase: This enzyme, can not adsorb on raw starch, assists the raw starch digestion by Rhizopus glucoamylase I. 4. Beta-amylases 1) Plant β-amylase can not adsorb on raw starch and has no activity to digest raw starch. 2) Bacterial β-amylase can adsorb on raw starch and has a strong activity to digest raw starch.

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