Abstract
AbstractThe glucoamylase system of Rhizopus sp. was fractionated into two kinds of glucoamylase by using the corn starch adsorption technique supplemented by CM‐cellulose chromatography. One of these fractions, referred to as glucoamylase I, has a strong debranching activity and is highly active in raw starch digestion.The raw waxy corn starch digestion by glucoamylase I was accelerated by adding a‐amylase from Rhizopus sp. or from Aspergillus oryzae. The raw non‐waxy corn starch digestion by glucoamylase I was more difficult than the raw waxy corn starch digestion, which was also accelerated by adding α‐amylase from Aspergillus oryzae.On the other hand, another fraction, referred to as glucoamylase II, has a weak debranching activity and can not be adsorbed on raw starch. The raw waxy corn starch digestion by glucoamylase II was very weak, but was accelerated by adding α‐amylase from Rhizopus sp.
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