铝胁迫下胡枝子根尖胼胝质形成规律及影响因素

Abstract

PDF HTML阅读 XML下载 导出引用 引用提醒 铝胁迫下胡枝子根尖胼胝质形成规律及影响因素 DOI: 10.5846/stxb201406091185 作者: 作者单位: 中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室,湖北理工学院环境科学与工程学院,中国科学院南京土壤研究所土壤与农业可持续发展国家重点实验室 作者简介: 通讯作者: 中图分类号: 基金项目: 土壤与农业可持续发展国家重点实验室开放基金课题(0812201225);湖北省自然基金面上项目(2014CFC1089);湖北省矿区环境污染控制与修复重点实验室开放基金(2013104);湖北理工学院引进人才项目(10yjz04R);国家重点基础研究发展计划项目(973项目)(2014CB441000) Analysis of variations in and factors affecting callose formation in response to al stress in Lespedeza Root Tips Author: Affiliation: State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,College of Environmental Science and Engineering,Hubei Institute of Technology,Huangshi,State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences Fund Project: 摘要 | 图/表 | 访问统计 | 参考文献 | 相似文献 | 引证文献 | 资源附件 | 文章评论 摘要:采用水培试验,研究了铝胁迫下两个胡枝子品种根尖产生胼胝质的变化规律及影响因素。结果表明,两个品种的根尖铝吸收量与胼胝质形成量呈正比例关系。品种间差异主要是在根尖0-0.5 cm处。敏感品种胼胝质形成量同铝吸收量的变化趋势相一致,而耐性品种则在铝处理6 h时出现一个高峰值后下降。去除铝胁迫后,耐性品种胼胝质形成量并不显著减少。与单独铝处理相比,阴离子通道抑制剂苯甲酰甲醛加铝处理对两个品种胼胝质形成无影响;尼氟灭酸加铝处理抑制敏感品种胼胝质的形成,对耐性品种无影响;蒽-9-羧酸加铝处理显著抑制两个品种的胼胝质形成。另外,抑制剂2-去氧-D-葡萄糖加铝共同处理与单独铝处理相比,敏感品种的胼胝质形成量显著降低,耐性品种无影响。甘露醇对两个品种胼胝质形成的影响无显著差别。镧处理下胼胝质的形成量是耐性品种显著高于敏感品种,铝、镧同时处理胼胝质的形成量最高。敏感品种胼胝质形成处理间无差别。总之,耐性品种在铝胁迫下胼胝质形成与有机酸分泌可能存在一定的协调关系;铝胁迫下胼胝质形成是敏感指标;在一定条件下,特别是有机酸分泌前胼胝质的形成可能具有一定抗性意义;铝诱导胼胝质的形成受多种外界因素(浓度、时间、有机酸分泌,渗透压等)的影响。 Abstract:Lespedeza speices can grow very well in infertile acidic soils as native pioneer plants. The reasons why such plants are well adapted to these soils might be due to a combination of mechanisms. Al toxicity is the primary factor limiting plant growth in acidic soils. Our previous results indicated that secretion of malate and citrate from roots was related to the high Al-resistance of Lespedeza. The objective of this study was to investigate the formation of callose under Al stress and its regulating factors in two Lespedeza species with different Al resistance, and attempted to explore the relative role of callose formation and organic acid exudation in Al toxicity or resistance of Lespedeza. The results showed that increased Al uptake was associated with increased callose formation in both Lespedeza species. The differences in Al tolerance between the two species were mainly expressed at the root apical 0-0.5 cm. Moreover, the callose formation was similar to root Al uptake for Al-sensitive species, but not for Al-tolerant species, of which callose formation peaked at 6 h of Al treatment and then decreased. Callose formation of the tolerant species was not obviously reduced following removal of Al from the culture. Furthermore, no obvious differences in callose formation were observed under treatment with PG (phenylglyoxal, anion-channel inhibitor) plus Al as compared to Al treatment alone in both species. NIF (niflumic acid, anion-channel inhibitor) plus Al treatment inhibited callose formation in the Al-sensitive species, but not in the Al-tolerant species. Callose formation was inhibited by A-9-C (9-anthracene carboxylic acid, anion-channel inhibitor) plus Al treatment in both species. Compared with Al treatment alone, DDG plus Al treatment caused a significant reduction in callose formation in the Al-sensitive species but not in the Al-tolerant species. No differences in callose formation were observed for both species following mannitol treatment. Callose formation in response to La treatment was significantly higher in the Al-tolerant species than in the Al-sensitive species and was still higher following treatment with both Al and La. However, no differences in callose formation in Al-sensitive species were detected among treatments. In summary, callose synthesis may be regulated in conjunction with organic acid exudation. Callose formation may be a sensitive indicator of Al stress and may also function in Al tolerance before organic acids are secreted from roots. Finally, callose formation may be dependent on many factors, including Al concentration, treatment time, organic acid exudation, and osmotic pressure. Further studies are required to examine these additional factors to determine the complex mechanisms mediating callose formation. 参考文献 相似文献 引证文献