大豆愈伤组织超弱光子辐射的双指数模型

Abstract

Double-exponential model of ultraweak photon emission of soybean callus was proposed based on the mechanism of ultraweak photon emission in biological system. Treated soybean callus by UV-B radiation (20 μW/cm^2) for 2 h, ultraweak photon emission of soybean callus treated by UV-B for 4 d after was measured. The results show that double-exponential model accurately describes the characteristics of ultraweak photon emission of soybean callus and its changes, delayed luminescence of ultraweak photon emission of soybean callus is composed by both fast and slow phase. Spontaneous luminescence in ultraweak photon emission of soybean callus and some parameters of delayed luminescence such as initial photon number I(0), integrated intensity I(T) and decay parameters of delayed luminescence are obtained through analysis of the double-exponential model, the characteristics of ultraweak photon emission of soybean callus can be quantitative expressed by these parameters. The studies also find that there is an excellent positive correlation between initial photon number I(0) and integrated intensity I(T), and I(0) or I(T) can be used to measure the metabolic intensity of soybean callus. Induced by UV-B radiation (20 μW/cm^2) for 2 h, the decay parameter of fast phase in delayed luminescence decreases first and then increases, the change of spontaneous luminescence is opposite, it suggests that fast phase of delayed luminescence is sensitive to the UV-B radiation and membrane lipid peroxidation could be the reason of the drastic change of fast phase in delayed luminescence of soybean callus.