Abstract
Four cultivars (‘Arendsoog’, ‘Beatrix’, ‘Continent’ and ‘Super Giant Yellow’) of gerbera were used in this experiment.The flower stem, also referred to as scape, was used in tissue culture. When the 2nd or 3rd whorl of the disc florets started to unfold, the upper 4cm solid part of the scape just below the head was cut off and sterilized for 2 or 3 seconds in 70% ethanol, followed by soaking in 2% NaOCl for 30 minutes, and then rinsed 3 times with sterilized distilled water. After sterilization, both ends of the scape section were removed 0.5cm in length and discarded. It was divided longitudinally into identical strips. Each strip was placed with the wounded side down on the culture medium composed of MS (Murashige and Skoog, 1962) macroelements at 1/2 strength, Heller′s microelements, Na2FeEDTA 21.4mg/l, MS organic constituents, 1% sucrose, 6-benzyl amino purine. (BA) 10mg/l and Bacto-agar 0.8%. The pH of all nutrient media was adjusted to 5.6 prior to autoclaving.The explants were first maintained in complete darkness for 2 weeks, and then subjected to light at intensity of 800lx for 16 hours daily. At the end of 16 weeks, 5 or more shoots were formed per scape.
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