Abstract
Bacteriocidal effect of carbon dioxide laser irradiation was quantified as a function of the laser output (P), exposure time (t), laser beam spot size (φ) and base temperature (T) of the target (culture media). An Aloka's surgical CO2 laser was used for defocused (φ 6, 12mm) irradiations of the laser beam onto staphylococci-inoculated, modified Müller-Hinton culture plates. Samples were incubated at 37°C for 20 hours.The size of clear, circular zones of bacterial growth inhibition was found to have a functional relationship with the dose of laser irradiation (power×exposure time) and also depend on the beam spot size as well as the temperature of the target.The experimental data also indicated that the values of the laser power and the exposure time for threshould inhibition satisfies the following equations: P=k/√t and ΔT=KP√t, where k and K are coefficients related to the target temperature and ΔT is the maximal temperature rise at the irradiated center of the inoculated media.
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