Abstract
Previous reports indicated that the histological intensity of the tumor infiltrating lymphocytes (TIL), which is one of the manifestations of immunological host's response against malignant tumor cells, correlates with the clinical prognosis of the cancer patients.In the present study, we investigated the antitumor cytotoxicity of TIL (or LN-TIL: lymphocytes of metastatic lymph node) induced by anti-CD3 antibody and interleukin 2 (IL-2), and the availability for the source of the adoptive immunotherapy on patients with oral malignant tumors.TIL were isolated from 28 tumors and LN-TIL were isolated from 6 metastatic lymph nodes obtained from 28 patients with oral malignant tumors.Natural killer (NK) and lymphokine-activated killer (LAK) activity of freshly isolated TIL were usually undetectable. However the antitumor cytotoxicity of TIL was generated with IL-2 in vitro culture. These activated TIL showed high levels of NK and LAK activities, and these were prolonged more than those of PBL.On the other hand, freshly isolated TIL from patients who received IL-2 injection directly into the tumor lesions before surgery, showed NK and LAK activities.The growth of TIL were obviously enhanced by using the plates coated with anti-CD3 antibody at the beginning of the culture.These activated TIL (or LN-TIL) with anti-CD3 antibody and/or IL-2 also showed the cytotoxicity against the autologous tumor cells.These results indicate that anti-CD3 antibody and IL-2 augment the specific and/or nonspecific cytotoxicity of the TIL (or LN-TIL) against the tumor cells, and this augmentation of cytotoxic activity shows the anti-tumoral efficacy for oral malignant tumors. Furthermore, TIL (or LN-TIL) from oral malignant tumors could be on appropriate effector cells for adoptive immunotherapy on the patients with oral malignant tumors.
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call