Abstract
Growth retardation and diseases hindering growth are constraints faced by Macrobrachium rosenbergii and thus urge an in-depth study on the growth and growth-related genes to improve this species. This study focused on molecular cloning and characterization of the myostatin gene in M. rosenbergii (MrMSTN) followed by mRNA transcription in different life stages and upon infectious hypodermal and hematopoietic necrosis virus (IHHNV) infection. We have characterized a full-length MrMSTN gene encoding 1619 base pairs (bp) with an open reading frame of 945 bp that encodes for 315 amino acid residues. MrMSTN retains all the conserved characteristics belonging to TGF-β superfamily including propeptide and mature peptide domain, cysteine residues and a proteolytic cleavage site, RXXR where “XX” denotes Asparagine and Arginine. MrMSTN gene is ubiquitously expressed in all the tissues of healthy adults, with the highest expression observed in muscle. Moreover, the MrMSTN transcripts showed significant (P < 0.05) changes in different life stages of M. rosenbergii and IHHNV-challenged prawns. The results indicate the possible functional role of MrMSTN as a negative growth regulator. Thus, MrMSTN can be exploited further to enhance the growth and can be used as a biomarker to address the growth deficiency-related problems in M. rosenbergii.
Published Version
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