Abstract
Objective To study the myocardial protective effect of micro-flow perfusion with blood Plegisol solution on warm ischemic pig heart and the mechanism.Methods Twelve isolated pig hearts were subjected to orthotopic heart transplantation and randomly divided into experimental group (n =6,warm ischemia for 10 min,4℃ blood the micro-flow continuous infusion for 8 h,and warm-blooded continuous perfusion during transplantation) and control group (n =6,wark ischemia for 10 min,and immersion with 4℃ Plegisol liquid alone for 8 h).The pathological changes of myocardial tissue in all pig hearts after reperfusion were observed.The superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in myocardial tissue were measured by xanthine oxidase and thiobarbituric acid method.Immunohistochemistry was used to detect B lymphocytes/leukemia-2 (bcl-2) and Caspase-3 protein expression.Results In control group,coronary endothelial and myocardial tissue injury was more serious than in experimental group.As compared with control group,myocardial tissue SOD activity [(7.88 ± 0.41) U/(mg· protein)] was significantly increased,and MDA content [(9.97 ± 1.05) nmol/(mg· protein)] was significantly reduced in experimental group (P ＜ 0.05).The expression levels of bcl-2 mRNA and protein in experimental group (0.651 ±0.052 and 0.506 ±0.048) were significantly increased as compared with control group (0.337 ±0.026 and 0.271 ±0.022),and those of Caspase-3 mRNA and protein in experimental group (0.343 ± 0.027 and 0.215 ± 0.019) were significantly decreased as compared with control group [(0.782 ±0.059 and 0.629 ±0.054),P ＜0.01].Conclusion The mechanism by which micro-flow perfusion with blood Plegisol solution protects warm ischemic pig heart may be related to the alleviation of the isolated pig heart coronary endothelial and myocardial edema,reduction of the reperfusion oxidative damage and the inhibition of cardiac apoptosis. Key words: Micro-flow infusion; Warm ischemic; Myocardial protective
Published Version
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