Mycosynthesis of ultrasonically-assisted uniform cubic silver nanoparticles by isolated phenols from Agaricus bisporus and its antibacterial activity

Abstract

The present study focuses on a facile and environmentally friendly process to synthesize uniformly cubic-shaped silver nanoparticles (AgNPs) with ultrasonic assist in a short time. The phenolic compounds isolated from edible mushroom (Agaricus bisporus) were employed in the mycosynthesis of cubic AgNPs for antibacterial properties. Here, for the first time, the ultrasonic instrument was used to isolate phenols as a strong reducing agent and synthesize cubic AgNPs. This method of particle preparation revealed that the AgNPs are in uniform cubic shape and small size distribution compared to literature. TEM and FESM results displayed the cubic sizes with an average cube diameter of 50.44 nm and fewer sharp tips. The UV–Vis spectrum of AgNPs exhibited two maximum absorption peaks (SPR) at 401 and 546.5 with a broad absorption spectra area from almost 400 to 639 nm. The EDX and XRD proved the formation of AgNPs and a single-phase Ag with fcc structure. HPLC results analysis indicated that the AgNPs are surrounded by phenol compounds which act as a stabilizer agent and increase the antibacterial activity. Mycosynthesized AgNPs produced significantly enhanced in inhibiting the growth zone of Pseudomonas aeruginosa (P. aeruginosa) and Staphylococcus aureus (S. aureus).