Abstract

This report will briefly review our current knowledge of: 1) the incidence, prevalence and sources of mycoplasma contamination in cell cultures; 2) the procedures for isolation, detection and identification of mycoplasmas, including procedures recommended for testing biological products produced for human use; 3) the effects of mycoplasma contamination and /or infection on the function and activities of various infected cell cultures; and 4) the recommended procedures for prevention and elimination of mycoplasma contamination. Extensive reviews on mycoplasma contamination in cell culture have been reported earlier (Barile, 1979; DelGiudice and Hopps, 1978; McGarrity and Kotani, 1985).

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