Mycoplasma gallisepticum transmission: Comparison of commercial F-strain vaccine versus layer complex-derived field strains in a tunnel ventilated house

Abstract

Two simultaneous trials were conducted using a commercially available, live, F strain Mycoplasma gallisepticum (FMG) vaccine (trial 1) or 2 inocula of layer complex-derived MG strains (LCD-MG; trial 2). In each of the 2 trials, 4 commercial turkeys were housed in each of 2 adjoining pens immediately adjacent to air inlets. The turkeys (8/trial) were inoculated in the right eye with either a 1× dose of FMG (trial 1) or with 0.02 mL of 1 of 2 actively growing LCD-MG inocula (4 turkeys/inoculum; trial 2). In each of the 2 trials, one pen housing 4 inoculated turkeys was maintained without the addition of other poultry, whereas 16 MG-free broilers and 4 MG-free layers were added to the other pen of 4 inoculated turkeys. Within each of the trials and at increasing intervals, either 4 layers (3 pens) or 4 turkeys (3 pens) were placed down-airstream from the inoculated pens. The distance of the first pen from the inoculated turkeys was separated by the width of one pen that was empty. Succeeding down-airstream pens were situated such that the empty distance (absence of any poultry) between pens that contained poultry doubled from one pen to the next such that the final pen that contained poultry had 4 empty pens between it and the next up-airstream pen that also contained poultry. At 106 d postinoculation, all poultry were bled, swabbed for MG from the choanal cleft, and then euthanized and necropsied. No commingled poultry in trial 1 (FMG), whether inoculated (turkeys) or commingled (layers and broilers), died during the course of the trial, and 5 of the 8 FMG-vaccinated turkeys exhibited serological but not cultural evidence of mycoplasmosis. In trial 2 (LCD-MG), 2 commingled broilers died and no inoculated turkeys exhibited either serological or cultural evidence of mycoplasmosis. In both trials, no poultry housed down-airstream from the inoculated poultry showed evidence of clinical signs of mycocplasmosis and none showed either serological or cultural evidence of mycoplasmosis.