Mycoflora Associated with Black Point Attack on Two-row and Six-row Barley - First Report

Abstract

The research followed the signaling of the black point attack on two-row and six-row barley, the identification of the associated mycoflora and the establishment of the average seed infection rate. The biological material was represented by six-row barley from the Cardinal variety cultivated in the conventional system and during the conversion period, the Gerlach variety and Romanita barley in two-rows variety. The experiments were carried out under in vitro conditions with non-disinfected seed variants and subjected to a light disinfection with sterile water and 70% ethanol solution. The black point attack on the analyzed seeds induced a chocolate brown to black color in the embryonic area and the sector of associated fungal microorganisms was made up of species Alternaria spp., Fusarium spp., Drechslera spp., to which Aspergillus spp. was added, Penicillium spp. The average seed infection rate with Alternaria spp was 46.66 in the Cardinal and Romanita varieties and 40% with Fusarium spp. in the Gerlach variety. The average seed infection rate with Drechslera spp. was 20% in the Cardinal and Romanita varieties. When disinfecting with 70% ethanol around the seeds, colonies of Alternaria spp. and Fusarium spp. developed well.