Mycobiota of <em>Lupinus albus</em> seeds from a public germplasm collection

Abstract

Seedborne mycobiota of Lupinus albus was assessed using blotter paper and agar media with Rose Bengal or semi-selective media for Pythium or Fusarium . Samples of 200 seeds were taken from each of 16 inventories, comprising 14 accessions originating from Germany, France, Ukraine, Syria, Hungary or Spain, and increased 1-2 times through propagation by the USDA-ARS Western Regional Plant Introduction Station. A total of 307 fungus isolates were recovered and preserved with over 96% belonging to 10 genera: Penicillium (47%), Cladosporium (32%, including Cladosporium -like Chalastospora ) , Alternaria (4%), Aspergillus (4%), Fusarium (3%), Rhizopus (3%) , Ulocladium (3%), Chaetomium (0.5%) and Arthrinium (< 0.5%). By sub-sampling, fungi were further identified by morpho-cultural characters and/or DNA sequences as Alternaria infectoria , A. alternata, and A. tenuissima species-groups, Arthrinium arundinus , Aspergillus niger , Chalastospora gossypii , Cladosporium herbarum and C. cladosporioides species complexes, Chaetomium sp., Fusarium acuminatum , Rhizopus stolonifer , and Ulocladium atrum group. Penicillia included P. hordei , but most Penicillia could not be assigned species names either by morphological traits or β-tubulin DNA sequence data.