Mycobacterium tuberculosis drug resistance comparison between Nitrate Reducrase Assay and Applicating Line Probe Assay

Abstract

Objective To establish a joint detection method of Mycobacterium tuberculosis drug resistance,and improve the sensitivity and specificity of drug resistance detection methods.methodsTaking absolute concentration method for drug susceptibility testing of Mycobacterium tuberculosis as a “golden standard,” examined the Mycobacterium tuberculosis drug resistance and sensitivity to rifampicin (RFP),isoniazid (INH),streptomycin (SM),and ethambutol (EMB) with Nitrate Reducrase Assay(NRA) and Applicating Line Probe Assay(LiPA).Results Comparing the nitrate reduction test with absolute concentration method,the drug resistance coincident rate of INH was 77.6％,and its sensitivity coincident rate was 86.6％; the drug resistance coincident rate of RFP was 95.8％,and its sensitivity coincident rate was 92％; the drug resistance coincident rate of SM was 84.1％,and its sensitivity coincident rate was 100％; the drug resistance coincident rate of EMB was 94.1％,and its sensitivity coincident rate was 85.7％.Comparing linear DNA hybridization with absolute concentration method,the drug resistance coincident rate of INH was 89.6％,and its sensitivity coincident rate was 93.3％; the drug resistance coincident rate of RFP was 98.6％,and its sensitivity coincident rate was 100％; the drug resistance coincident rate of SM was 78.3％,and its sensitivity coincident rate was 100％; the drug resistance coincident rate of EMB was 88.2％,and its sensitivity coincident rate was 100％.Comparing the nitrate reduction test with linear DNA hybridization,the drug resistance coincident rate of INH was 80.6％,and its sensitivity coincident rate was 82.9％; the drug resistance coincident rate of RFP was 100％,and its sensitivity coincident rate was 100％; the drug resistance coincident rate of SM was 97.2％,and its sensitivity coincident rate was 96.7％ ; the drug resistance coincident rate of EMB was 100％,and its sensitivity coincident rate was 83.5％.Conclusion Both nitrate reduction test and linear DNA hybridization can be used as a fast detection method of Mycobacterium tuberculosis drug resistance.They are coincident. Key words: M. tuberculosis; drug resistance; Nitrate Reducrase Assay; Applicating Line Probe Assay