Abstract
As an important nongrain crop, the growth and yield of potato (Solanum tuberosum L.) is often affected by an unfavorable external environment in the process of cultivation. The MYB family is one of the largest and most important gene families, participating in the regulation of plant growth and development and response to abiotic stresses. Several MYB genes in potato that regulate anthocyanin synthesis and participate in abiotic stress responses have been identified. To identify all Solanum tuberosum L. MYB (StMYB) genes involved in hormone or stress responses to potentially regulate potato growth and development, we identified the MYB gene family at the genome-wide level. In this work, 158 StMYB genes were found in the potato genome. According to the amino acid sequence of the MYB domain and gene structure, the StMYB genes were divided into R2R3-MYB and R1R2R3-MYB families, and the R2R3-MYB family was divided into 20 subgroups (SGs). The expression of 21 StMYB genes from different SGs in roots, stems, leaves, flowers, shoots, stolons, young tubers, and mature tubers was determined by quantitative real-time polymerase chain reaction (qRT-PCR). The expression patterns of StMYB genes in potatoes treated with abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellin acid 3 (GA3), NaCl, mannitol, and heat were also measured. We have identified several potential candidate genes that regulate the synthesis of potato flavonoids or participate in hormone or stress responses. This work provides a comprehensive understanding of the MYB family in potato and will lay a foundation for the future investigation of the potential functions of StMYB genes in the growth and development of potato.
Highlights
The abiotic stresses that plants encounter in their growth process seriously affect their growth and productivity
Several MYB genes involved in stress response have been identified in wheat, among which TaMYB1 can respond to abiotic stress and abscisic acid (ABA) treatment, TaMYB2A in transgenic Arabidopsis thaliana (L.) Heynh. showed a variety of abiotic stress tolerances, while TaMYB33 can reconstruct the osmotic balance of transgenic plants, thereby improving their salt tolerance and drought tolerance [20,21,22]
The molecular weights (MWs) of these Solanum tuberosum L. MYB (StMYB) proteins ranged from 5.89 kDa (StMYB26) to 113.39 kDa (StMYB146), and their PIs ranged from 4.59 (StMYB78) to 10.26 (StMYB10)
Summary
The abiotic stresses that plants encounter in their growth process seriously affect their growth and productivity. According to the conserved domains of MYB genes and their functions, the R2R3-MYB family in Arabidopsis thaliana (L.) Heynh. Studies have shown that R2R3-MYB family genes play an important role in the plant responses to abiotic stress, besides participating in various physiological activities. Several MYB genes involved in stress response have been identified in wheat, among which TaMYB1 can respond to abiotic stress and ABA treatment, TaMYB2A in transgenic Arabidopsis thaliana (L.) Heynh. Sci. 2019, 20, 4847 growth, development, and stress response were excavated Their motif composition, intron and exon distribution, and tandem duplication and segmental duplication events were comprehensively analyzed. By determining the expression patterns of StMYB genes under ABA, IAA, GA3, NaCl, mannitol, and high temperature treatments, we identified some StMYB genes that may potentially play a vital role in potato growth and hormone stress resistance. This study can provide a meaningful reference for potato breeding improvement
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