Abstract
Background The harmonization of action of enzymes and non-enzymatic compounds is poorly understood. Objective To explore interaction among enzymatic and nutrient markers of the antioxidant defense system in blood with urinary biomarkers of oxidative stress in low-income Guatemalan children consuming a common menu offering. Methods: Whole blood and 24-h urines were collected from 82 children, 2-7y, from the Western Highlands. Urinary oxidative stress biomarkers included F2-isoprostanes (F2-Iso) and 8-OHdG. Red cell enzyme activities were: catalase; superoxide dismutase; glutathione peroxidase and glutathione reductase. Circulating antioxidant nutrients included: retinol, α-tocopherol, β-carotene and coenzymes Q9 and Q10. Results: 28 (51%) of 55 paired combinations of the 11 biomarkers were significantly and mutually correlated (p蠄0.05), most strongly between retinol and α-tocopherol (r=0.697, p<0.001). F2-Iso showed the greatest number of cross-associations, having significant interactions with 8 of the 10 remaining biomarkers. Backward-elimination multiple regression indicated that plasma retinol, β-carotene and coenzyme Q10 were independent predictors of urinary F2-Iso. Conclusions The antioxidant enzymes and antioxidant nutrient mitigation of oxidative stress works in concert to deal with daily life prooxidative challenges. Funding: Hildegard Grunow Foundation.
Published Version
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