Abstract
Seeds of <em>Lathyrus sativus</em> cv. Derek and Krab were used as biological material for induced mutagenesis. Three mutant lines were obtained from seeds of grass pea cv. Derek and 15 lines from mutagenised seeds of cv. Krab. Twelve ethanol-soluble carbohydrates were identified in the seeds. We have selected grass pea mutant lines with high oligosaccharides content (lines D4, K56, K25, and K7) and lines with low raffinose family oligosaccharides (RFO) content (lines K12, K29 and K13). Mutations changing the levels of RFO have not affected the contents of galactosyl cyclitols.
Highlights
Lathyrus is a large genus within Leguminosae (= Fabaceae) family, subfamily Papilonoideae, tribe Vicieae, containing 187 species and subspecies that are found in both the Old World and the New World (Allkin et al 1983; Campbell 1997)
Three mutant lines were obtained from seeds of Lathyrus sativus L. cv
Seeds of mutant line D11 of cultivar Derek had soluble carbohydrates level similar to non-mutagenised seeds, while in seeds of D4 line the level of these compounds was 245 mg/g d.m. (Table 1), so it increased five-fold compared to control
Summary
Lathyrus is a large genus within Leguminosae (= Fabaceae) family, subfamily Papilonoideae, tribe Vicieae, containing 187 species and subspecies that are found in both the Old World and the New World (Allkin et al 1983; Campbell 1997). Lathyrus species have a considerable potential in crop rotation, improving soil physical conditions, limiting pathogen and weed populations, and reducing overall production costs (Vaz Patto et al 2006). For these reasons grass pea is a major crop in Bangladesh, China, India and Pakistan and it is cultivated on a smaller scale in many countries of Europe, the Middle East, Northern Africa, as well as in Chile, Brazil and other countries of South America (Campbell et al 1994). Grass pea was already in use in Neolithic times, and presently is considered a model crop for sustainable agriculture
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