Abstract
A full length α-subunit of the Ca2+-activated K+(BK) channel with an inactivating mutation in the C-terminus can complement a functional C-terminal fragment. We analyzed deletions and amino acid changes within the S8–S9 interdomain region for their ability to allow complementation. Cys612and His616that are located in a region that contains two overlapping signature sequences, a immunoglobulin signature sequence and a heme binding domain, are essential for a functional channel. These two amino acid residues are also essential for complementation. The deletion of the PEST sequence does not affect the function of the BK channel; however, without the PEST sequence, complementation by a functional C-terminal fragments is no longer possible. The ability to complement a functional channel is restricted to the C-terminal fragment and requires that the complete α-subunit or the larger N-terminal fragment contains both, the immunoglobulin signature sequence the PEST sequence.
Published Version
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