Abstract
By the treatment of E. coli Q13 with a mutagen, 76 kanamycin-resistant mutants were isolated. Of them, five mutants, showing various degrees of cross-resistance to neomycin, gentamicin or streptomycin, were studied in detail. The degree of inhibition by kanamycin of polyphenylalanine synthesis was less on the ribosomes derived from the resistant mutants than on the parental ribosomes. The mutant ribosomes exhibited less affinity for [ 3H]kanamycin than the sensitive ribosomes. The effect of the antibiotic on polypeptide synthesis with the hybrid ribosomes, consisting of the resistant and parental subunits, and the binding of [ 3H]kanamycin to the subunits suggested that the resistance is attributed to the 30S ribosomal subunits in the three mutants, and to the 50S subunits in the other two mutants.
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