Mutation of YL Results in a Yellow Leaf with Chloroplast RNA Editing Defect in Soybean.

Xiaowei Zhu,Zongbiao Duan,Yi Pan,Zhixi Tian,Zhi Liu,Guoan Zhou,Deyi Zhong,Yucheng Liu,Baoge Zhu

doi:10.3390/ijms21124275

Abstract

RNA editing plays a key role in organelle gene expression. Little is known about how RNA editing factors influence soybean plant development. Here, we report the isolation and characterization of a soybean yl (yellow leaf) mutant. The yl plants showed decreased chlorophyll accumulation, lower PS II activity, an impaired net photosynthesis rate, and an altered chloroplast ultrastructure. Fine mapping of YL uncovered a point mutation in Glyma.20G187000, which encodes a chloroplast-localized protein homologous to Arabidopsis thaliana (Arabidopsis) ORRM1. YL is mainly expressed in trifoliate leaves, and its deficiency affects the editing of multiple chloroplast RNA sites, leading to inferior photosynthesis in soybean. Taken together, these results demonstrate the importance of the soybean YL protein in chloroplast RNA editing and photosynthesis.

Highlights

IntroductionChlorophyll a and b are the two major pigments located in the thylakoid membrane of photosynthetic organisms [2]
Photosynthesis is a vital process in which plants convert light energy into chemical energy [1,2].In higher plants, chlorophyll a and b are the two major pigments located in the thylakoid membrane of photosynthetic organisms [2]
We investigated the distribution of the affected editing sites in the yl mutant

Summary

Introduction

Chlorophyll a and b are the two major pigments located in the thylakoid membrane of photosynthetic organisms [2]. These chlorophyll molecules play essential roles in harvesting light energy and transferring that energy to reaction centers of the photosystem [2,3]. 15 chlorophyll-deficient mutant genes were reported to be mapped to chromosomes [4]. Of these genes, six lethal yellow mutant genes, including Y11, Y18/Y18_1, Y18_2, YL_PR350, PsbP, and CD-5, were mapped to chromosomes 13, 14, 17, 15, 3 and 15, respectively [5,6,7,8,9]

Methods

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Discussion

Conclusion