Mutation Analysis of the pKa Modulator Residue in β-D-xylosidase from Geobacillus Thermoleovorans IT-08: Activity Adaptation to Alkaline and High-Temperature Conditions

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β-D-xylosidases are hemicellulases that catalyze the release of xylose units from short xylooligosaccharides. Their activity is the rate-limiting step in xylan hydrolysis. A major application of these enzymes is as eco-friendly biobleaching agents in the pulp and paper industry, where thermostable, alkaliphilic xylosidases are much preferred. Hence, the isolation and characterization of new thermo-alkaliphilic xylosidases from nature, or re-engineering existing ones is of importance for industrial applications. The thermophilic bacterium Geobacillus thermoleovorans IT-08 produces a meso-thermophilic β-D-xylosidase (EC 3.2.1.37), which has a unique primary structure compared to other xylosidases with only 32-35% amino acid sequence identity. Here, we describe the role of Asp121 in modulating the enzyme's pH-activity profile. Mutation of this residue into Val121 or Asn121 increased its pH optimum by 3 pH units with a significant decrease in its activities. Furthermore, all mutants showed a significant 40°C increase in optimum temperature compared to wild-type enzyme. This study thus illustrates the importance of the pKa modulator residue Asp121 in shifting the pH- and temperature-activity profile of the enzyme. Changing Asp/Asn residues in enzymes, especially when the residue is located near the catalytic site, may be a useful strategy for adapting the enzyme to a high pH and high temperature environment.