Abstract
The present study was aimed to investigate the effect of different doses (0.2%, 0.4%, 0.6% and 0.8%) of sodium azide (SA) on M2 generationof Brassica napus (variety Dunkled) on some qualitative, quantitative and biochemical parameters. The result showed that days to germination, days to flowering and days to siliqua maturation were delayed (9.4, 98 and 153 days respectively) in higher treatment as compared to control (5.4, 96 and 147 days respectively). Higher concentration of SA (0.8%) decreased germination percentage (88.56%), plant height (97.95 cm), stem diameter (3.49 cm), number of branches/plant (3.49), number of leaves/plant (10), number of siliqua/plant (64.68), number of seed/siliqua (20.09) and 1000 seeds weight (3.91g) as compared to control. An increase was noticed for siliqua length (5.42 cm) in higher concentration of SA (0.8%) as compared to control (5.24 cm). Proximate analysis showed a significant decrease in oil, oleic acid and glucosinolates contents percentage (42.6%, 54.9%, and 58.5 μmol/g respectively) in higher concentration of SA (0.8%) as compared to control (44.7%, 58.1% and 71.3 μmol/g respectively) while a significant increase was noticed in proteins, moisture, linoleic acid and erucic acid percentage (28.4%, 5.9%, 9% and 37.3% respectively) in higher concentration of SA (0.8%) as compared to control (26.8%, 5.5%, 8.4% and 35.2% respectively. Keywords: Sodium Azide (SA); Brassica napus; morphological and biochemical parameters http://dx.doi.org/10.19045/bspab.2017.60018
Highlights
Introduction comprised of over338 genera and aboutRapeseed (Brassica napus) is known as3,710 species distributed worldwide [1].rape and Rapa
Materials and methods The seeds of M2 generation of Brassica napus variety Dunkled were obtained from the Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar
Germination percentage The results showed that there is no significant effect of various concentration of sodium azide on germination percentage
Summary
Materials and methods The seeds of M2 generation of Brassica napus variety Dunkled were obtained from the Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture, Peshawar. They were presoaked in distilled water for 4 to 6 hours at temperature 250C. Morphological parameter Various agronomic and morphological parameters were measured during the plants life span It includes the following: Days to germination, germination percentage, days to flowering, plant height, number of branches /plant, number of siliqua/plant, number of seeds/siliqua, number of leaves/plant, siliqua length, days to siliqua maturation and 1000 seeds weight Biochemical parameter Oil contents, protein contents, moisture contents, glucosinolates, oleic acid, linoleic acid and erucic acid Statistical analysis Experiment was set up in randomize complete block design with five replica per treatment.
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