Induction of Genetic Variability in Pusa Jwala and Pusa Sadabahar (Capsicum annuum L.) through Mutation via Sodium Azide and Hydroxyl Amine

Abstract

The present study was carried out to determine the mutagenic effect of Sodium Azide and hydroxyl amine on the morphological features of Pusa Jwala and Pusa Sadabahar (Capsicum annuum L.) in M2 generation. Seed were pre-soaked in distilled water for 6hrs and later treated with different concentration w/v (0.1, 0.15, 0.20, 0.25) of sodium azide and hydroxyl amine mutagens for 6hrs. The experiment was planted Randomized block design, replicated three times. Observation on growth parameters of M2 mutants of Pusa Jwala and Pusa Sadabahar shown significant variation on the plant height, germination, primary secondary branches, length of fruit, test weight of seed, number of fruits per plant, days to first flower mortality and survival. Most of the parameters decreased with increase in concentration of sodium azide. The result of study shown that sodium azide significantly affects plant height, germination and days to first flowering. Sodium Azide is more effective as compared to the Hydroxyl Amine in inducing genetic variability. Keywords: Chilli, Mutant, Sodium Azide, Hydroxyl Amine, Pusa Jwala, Pusa Sadabahar, Variability