Multiwavelength laser doppler holography (MLDH) for human retinal blood flow visualization and quantification in vivo

Abstract

We recently demonstrated high-speed, high-resolution structural imaging of the human eye in vivo by spatiotemporal optical coherence tomography (STOC-T). STOC-T extends the Fourier-Domain Full-Field Optical Coherence Tomography (FD-FF-OCT) by the spatial phase modulation to improve the imaging depth and suppress coherent noises. Here, we show that the dataset produced by STOC-T can be processed differently to reveal blood flow in the superficial and deep retina layers. Our method, denoted as multiwavelength LDH (MLDH) enables noninvasive visualization and quantification of the blood flow deep into the human retina at high speeds and high transverse resolution in vivo.