Abstract
BackgroundRapid increase in multi-walled carbon nanotube (MWCNT) production for their industrial and biomedical applications has led to concerns over the effects of MWCNTs on human health and the environment. Both animal and in vitro studies have provided important findings about MWCNT-induced effects on the lung cells or tissues. In vitro studies have provided a considerable amount of fundamental information on MWCNT-induced effects on the specific lung cells. However, the cell culture systems used in those studies were limited by the absence of dynamic nature of lung tissues. We hypothesized that MWCNT-induced cellular responses such as proliferation, inflammation, and oxidative stress under dynamic cell growth environment may differ from those under static cell growth environment.ResultsIn this study, we used a dynamic cell growth condition to mimic mechanically dynamic environment of the lung and characterized interleukin 8 (IL-8), reactive oxygen species (ROS), glutathione (GSH), and cell proliferation for three days following exposure of MWCNTs at different concentrations (5, 10, and 20 μg/ml) to A549 cell monolayer under both static and dynamic cell growth conditions. Our results demonstrated the distinct differences in the levels of inflammatory response and oxidative stress between static and dynamic cell growth conditions.ConclusionsIn conclusion, the dynamic cell growth system used in this study provided important changes in cellular responses that were not found in the static cell growth system and were similar to animal studies. The dynamic cell growth system can be considered as a viable alternative to in vivo test system in combination with existing in vitro static cell growth systems to evaluate the effect of MWCNTs on cellular responses in the respiratory system.
Highlights
Rapid advancement in the field of nanotechnology has given birth to various types of nanomaterials with unique mechanical, thermal, and electrical properties
Effect of multi-walled carbon nanotube (MWCNT) exposure on A549 cell growth Total protein concentration was measured at every 24 hour interval following exposure of MWCNTs at different concentrations (0, 5, 10, and 20 μg/ml) to A549 cell monolayer under static or dynamic cell growth conditions (Figure 2)
The dynamic cell growth system together with static cell growth system yielded several important findings: (1) All MWCNT exposure concentrations used in this study affected A549 cell proliferation
Summary
Rapid advancement in the field of nanotechnology has given birth to various types of nanomaterials with unique mechanical, thermal, and electrical properties. MWCNTs induce a variety of effects including increased inflammatory response, DNA damage, and cellular apoptosis in A549 cells (immortal human alveolar epithelial cell line), normal human bronchial epithelial cells and rat lung epithelial cells [15,16,17,18] These studies using specific cell type have provided abundant on the response of epithelial cells to external perturbations, but these systems are limited by the absence of dynamic nature of lung tissues [19]. Rapid increase in multi-walled carbon nanotube (MWCNT) production for their industrial and biomedical applications has led to concerns over the effects of MWCNTs on human health and the environment Both animal and in vitro studies have provided important findings about MWCNT-induced effects on the lung cells or tissues. We hypothesized that MWCNT-induced cellular responses such as proliferation, inflammation, and oxidative stress under dynamic cell growth environment may differ from those under static cell growth environment
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
