Abstract
This study aims at fabricating multiwalled carbon nanotubes (MWCNTs) based enzymatic bioelectrode for total cholesterol estimation in human serum. For this purpose, a gold (Au) electrode was modified with MWCNTs uniformly dispersed in nafion (Nf) matrix. Cholesterol oxidase (ChOx) and cholesterol esterase (ChEt) were immobilised onto this Nf–MWCNTs film-modified Au electrode using layer-by-layer technique to fabricate the final bioelectrodes. The immobilisation of ChOx and ChEt onto the electrodes was demonstrated by scanning electron microscopy. The fabricated bioelectrodes were electrochemically characterised using cyclic voltammetry. The bioelectrodes offer reliable response characteristics towards cholesterol and stable electrochemical properties in terms of extended linear response range of 0.080–0.950 mM, detection limit up to 0.01 mM and optimum storage stability up to three weeks. Experimental results reveal that the fabricated bioelectrode offer optimum repeatability and reproducibility towards the cholesterol estimation and can also efficiently exclude interference by the commonly coexisting ascorbic acid, uric acid, lactic acid, glucose and urea, which is favourable for its efficient use in the highly selective analysis of total cholesterol in serum samples.
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