Multispectral photoacoustic microscopy and optical coherence tomography using a single supercontinuum source

M Bondu,M.J Marques,P.M Moselund,G Lall,A Bradu,A Podoleanu

doi:10.1016/j.pacs.2017.11.002

M Bondu, M.J Marques + Show 4 more

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https://doi.org/10.1016/j.pacs.2017.11.002

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Journal: Photoacoustics	Publication Date: Nov 26, 2017
Citations: 22	License type: cc-by

Affiliation: University of Kent, Medway School of Pharmacy

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We report on the use of a single supercontinuum (SC) source for multimodal imaging. The 2-octave bandwidth (475–2300nm) makes the SC source suitable for optical coherence tomography (OCT) as well as for multispectral photoacoustic microscopy (MPAM). The IR band centered at 1310nm is chosen for OCT to penetrate deeper into tissue with 8mW average power on the sample. The 500–840nm band is used for MPAM. The source has the ability to select the central wavelength as well as the spectral bandwidth. An energy of more than 35nJ within a less than 50nm bandwidth is achieved on the sample for wavelengths longer than 500nm. In the present paper, we demonstrate the capabilities of such a multimodality imaging instrument based on a single optical source. In vitro mouse ear B-scan images are presented.

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Optical coherence tomography (OCT) and photoacoustic microscopy (PAM) provide complementary contrasts, which can be combined to benefit biomedical research and preclinical studies
In order to perform multispectral PAM (MPAM), one can use multiple lasers [12], optical parametric oscillators [13], stimulated Raman scattering fiber lasers [14,15] or supercontinuum sources [5,16,17]
We present spectroscopic photoacoustic measurements for different samples offering different absorption spectra in the visible as well as OCT-multispectral photoacoustic microscopy (MPAM) for different in vitro samples: synthetic phantoms and a mouse ear

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Introduction

Optical coherence tomography (OCT) and photoacoustic microscopy (PAM) provide complementary contrasts, which can be combined to benefit biomedical research and preclinical studies. PAM is based on tissue absorption properties by optical excitation and ultrasonic detection Both techniques offer real-time and non-invasive imaging. In order to perform multispectral PAM (MPAM), one can use multiple lasers [12], optical parametric oscillators [13] (at the cost of price and speed), stimulated Raman scattering fiber lasers [14,15] (discrete wavelengths with limited choice) or supercontinuum sources [5,16,17] (wide wavelength range with limited energy). Reported supercontinuum sources for MPAM delivered too little energy per pulse to image biological samples [16,17], more than 50 nJ within a bandwidth of less than 50 nm is typically required. Preliminary work targeting the enhancement in the pulse energy of supercontinuum sources (>100 nJ) has been reported [18,19]

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