Abstract
An efficient procedure for the simultaneous extraction and analysis of six fluoroquinolone (FQ) antibiotics is developed using an automated microdialysis-liquid chromatographic (LC) system. In this method, samples extracted from chicken liver and muscle are further purified by microdialysis, separated on an LC column, and the FQs detected by their fluorescence. Recoveries from fortified chicken liver and muscle samples are at least 70% with limits of quantitation (microg/kg) for the FQs in liver (and muscle) as follows: 0.3 (0.4) for danofloxacin, 0.8 (0.2) for desethylene ciprofloxacin, 2 (1) for norfloxacin, 2 (0.8) for enrofloxacin, 3 (1) for ciprofloxacin, and 5 (2) for sarafloxacin. Enrofloxacin and ciprofloxacin are determined in enrofloxacin-incurred chicken liver and muscle samples using this method.
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