MULTIPLIKASI TUNAS Begonia Salaziensis WARB. SEBAGAI UPAYA KONSERVASI TUMBUHAN TERANCAM PUNAH SECARA IN VITRO

Abstract

Begonia salaziensis Warb. is categorized critically endangered based on the IUCN Red List ver. 2.3. and need to be propagated to conserve. This study aims to investigate the effect of using BA and IAA plant growth regulator type and concentration on the growth and development of B. salaziensis culture. The study was arranged in a Completely Randomized Design (CRD) factorial consisting of 2 factors, i.e. the concentration of BA: 0 mg/l; 1 mg/l; 2 mg/l; and IAA concentration: 0 mg/l; 1 mg/l; 2 mg/l. Each treatment was repeated 10 times. To determine B. salaziensis germination the cultures were observed after 12 weeks. The results showed that the combination of BA with IAA on multiplication media had a significantly different effect on the number of shoot, average shoot height, and average number of shoot. The percentage of seed germination on media MS0 was 85%. The best multiplication of B. salaziensis shoots (9.3 ± 1.15) was obtained in MS media supplemented with 2 mg/l of IAA and 1 mg/l of BA. The lowest shoot multiplication (3.6 ± 0.84) was obtained on the control media (MS0) without addition of plant growth regulators, while the highest average length of shoots (1.87 ± 0.26 cm) was obtained on MS media with the addition 1 mg/l of IAA and BA 2 mg/l of BA. The lowest average length of shoots (0.67 + 0.25 cm) obtained on MS media with the addition of 2 mg/l of BA. The percentage of surviving planlets on acclimatization stage was 88.28%.